In vitro Dermal Absorption of Hydroquinone: Protocol Validation and Applicability on Illegal Skin-Whitening Cosmetics.
نویسندگان
چکیده
In Europe, hydroquinone is a forbidden cosmetic ingredient. It is, however, still abundantly used because of its effective skin-whitening properties. The question arises as to whether the quantities of hydroquinone used become systemically available and may cause damage to human health. Dermal absorption studies can provide this information. In the EU, dermal absorption has to be assessed in vitro since the Cosmetic Regulation 1223/2009/EC forbids the use of animals. To obtain human-relevant data, a Franz diffusion cell protocol was validated using human skin. The results obtained were comparable to those from a multicentre validation study. The protocol was applied to hydroquinone and the dermal absorption ranged between 31 and 44%, which is within the range of published in vivo human values. This shows that a well-validated in vitro dermal absorption study using human skin provides relevant human data. The validated protocol was used to determine the dermal absorption of illegal skin-whitening cosmetics containing hydroquinone. All samples gave high dermal absorption values, rendering them all unsafe for human health. These results add to our knowledge of illegal cosmetics on the EU market, namely that they exhibit a negative toxicological profile and are likely to induce health problems.
منابع مشابه
Qualitative and Quantitative Estimation of Hydroquinone in Skin Whitening Cosmetics
Hydroquinone has been used for decades as a skin lightening agent. Its use in cosmetics has been banned as a result of skin problems including contact dermatitis and ochronosis. A total of 22 samples of different skin whitening cosmetics were collected from local market. They were analyzed by using thin layer chromatography and HPLC for qualitative and quantitative determination of their hydroq...
متن کاملIn Vitro Methods for Predicting Chemical Leukoderma Caused by Quasi-Drug Cosmetics
Skin care cosmetics frequently contain whitening or lightening agents. The present study aimed to establish in vitro methods for predicting chemical leukoderma caused by whitening agents in cosmetics. The risks of chemical leukoderma were predicted based on percutaneous absorption rates, toxic concentrations, and toxicity mechanisms. Thus, in vitro skin permeation rate and cytotoxic concentrati...
متن کاملThe quest for a whiter shade of pale in skin care
The quest for a whiter shade of pale dates back to ancient civilizations, when a pale complexion was associated with aristocratic lineage. During the Tang dynasty (618-907), Chinese women used ground pearl powder as a whitening make-up. In ancient Persia, field workers bleached their skin with pure natural hydroquinone to keep it from darkening under the sun. In Europe, during the Renaissance, ...
متن کاملDetermination of the Thermodegradation of deoxyArbutin in Aqueous Solution by High Performance Liquid Chromatography
Tyrosinase is the key and rate-limiting enzyme responsible for the conversion of tyrosine into melanin. Competitive inhibition of tyrosinase enzymatic activity results in decreased or absent melanin synthesis by melanocytes in human skin. DeoxyArbutin (4-[(tetrahydro-2H-pyran-2-yl)oxy]phenol), a novel skin whitening agent, was synthesized through the removal of hydroxyl groups from the glucose ...
متن کاملPercutaneous absorption test – A case of effectiveness evaluation of skin-whitening cosmetics
To evaluate the effect of skin-whitening cosmetics, there is currently available a method to evaluate the degree of the disappearance of pigmented areas produced by ultraviolet irradiation by applying a sample to the skin. This method is convenient for evaluating a cosmetic formula. In animal models, this method shows much less individual differences, and due to the easiness in preparing animal...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Skin pharmacology and physiology
دوره 29 6 شماره
صفحات -
تاریخ انتشار 2016